8HVJ

The structure of the E. coli mRNA endoonuclease YiCC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	134.208, 77.158, 102.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.596 - 4.050
Rwork	0.321
R-free	0.38470
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.600	4.270
High resolution limit [Å]	4.050	4.050
Rmerge	0.135	0.630
Number of reflections	9159	1309
<I/σ(I)>	8.9
Completeness [%]	99.9	100
Redundancy	8.6	7.5
CC(1/2)	0.996	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	24.5% PEG 3350, 0.15M Ammonium sulfate, 0.1M HEPES pH7.5, 5mM MgCl2