8HVJ
The structure of the E. coli mRNA endoonuclease YiCC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 134.208, 77.158, 102.596 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 102.596 - 4.050 |
Rwork | 0.321 |
R-free | 0.38470 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.600 | 4.270 |
High resolution limit [Å] | 4.050 | 4.050 |
Rmerge | 0.135 | 0.630 |
Number of reflections | 9159 | 1309 |
<I/σ(I)> | 8.9 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.6 | 7.5 |
CC(1/2) | 0.996 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 24.5% PEG 3350, 0.15M Ammonium sulfate, 0.1M HEPES pH7.5, 5mM MgCl2 |