8HVC

Crystal structure of lacto-N-biosidase StrLNBase from Streptomyces sp. strain 142, galacto-N-biose complex 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-28
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	53.918, 50.852, 86.052
Unit cell angles	90.00, 97.92, 90.00

Refinement procedure

Resolution	48.400 - 1.580
R-factor	0.16968
Rwork	0.168
R-free	0.19865
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.579
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.350	1.610
High resolution limit [Å]	1.580	1.580
Rmerge	0.089	0.577
Rmeas	0.106	0.682
Rpim	0.058	0.360
Number of reflections	63448	10690
<I/σ(I)>	8.1	1.8
Completeness [%]	99.9	100
Redundancy	3.4	3.4
CC(1/2)	0.983	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.3 M Ammonium fluoride, 12.5%(w/v) PEG 3350