8HVC
Crystal structure of lacto-N-biosidase StrLNBase from Streptomyces sp. strain 142, galacto-N-biose complex 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-28 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.918, 50.852, 86.052 |
Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
Resolution | 48.400 - 1.580 |
R-factor | 0.16968 |
Rwork | 0.168 |
R-free | 0.19865 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.579 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.350 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.089 | 0.577 |
Rmeas | 0.106 | 0.682 |
Rpim | 0.058 | 0.360 |
Number of reflections | 63448 | 10690 |
<I/σ(I)> | 8.1 | 1.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.983 | 0.803 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.3 M Ammonium fluoride, 12.5%(w/v) PEG 3350 |