8HVB
Crystal structure of lacto-N-biosidase StrLNBase from Streptomyces sp. strain 142, lacto-N-biose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-21 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.052000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.925, 51.344, 86.507 |
| Unit cell angles | 90.00, 98.03, 90.00 |
Refinement procedure
| Resolution | 48.500 - 1.600 |
| R-factor | 0.1509 |
| Rwork | 0.149 |
| R-free | 0.18406 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.142 | 0.973 |
| Rmeas | 0.153 | 1.049 |
| Rpim | 0.057 | 0.391 |
| Number of reflections | 62063 | 3045 |
| <I/σ(I)> | 9.1 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.994 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.3 M Ammonium fluoride, 10%(w/v) PEG 3350 |
