8HVB

Crystal structure of lacto-N-biosidase StrLNBase from Streptomyces sp. strain 142, lacto-N-biose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-21
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.052000
Spacegroup name	P 1 21 1
Unit cell lengths	53.925, 51.344, 86.507
Unit cell angles	90.00, 98.03, 90.00

Refinement procedure

Resolution	48.500 - 1.600
R-factor	0.1509
Rwork	0.149
R-free	0.18406
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.585
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.142	0.973
Rmeas	0.153	1.049
Rpim	0.057	0.391
Number of reflections	62063	3045
<I/σ(I)>	9.1	3.1
Completeness [%]	100.0	100
Redundancy	7	7.1
CC(1/2)	0.994	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.3 M Ammonium fluoride, 10%(w/v) PEG 3350