8HUD
Cryo-EM structure of the EvCas9-sgRNA-target DNA ternary complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.43 |
| Number of Particle | 300599 |
| Software | cryoSPARC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
