8HU7

Crystal structure of FGF2-M1 mutant - D28E/C78L/C96I/S137P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97941
Spacegroup name	P 31 2 1
Unit cell lengths	54.375, 54.375, 97.012
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.090 - 1.400
Rwork	0.185
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.405
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1-2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.090	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.113
Number of reflections	33205	3023
<I/σ(I)>	64.3	5.2
Completeness [%]	99.8	99.3
Redundancy	14.6	9.4
CC(1/2)	1.000	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	289.15	1.5M sodium citrate, 100mM sodium cacodylate, pH 5.5