8HU7
Crystal structure of FGF2-M1 mutant - D28E/C78L/C96I/S137P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 54.375, 54.375, 97.012 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.090 - 1.400 |
| Rwork | 0.185 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.405 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1-2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.090 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.113 | |
| Number of reflections | 33205 | 3023 |
| <I/σ(I)> | 64.3 | 5.2 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 14.6 | 9.4 |
| CC(1/2) | 1.000 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 289.15 | 1.5M sodium citrate, 100mM sodium cacodylate, pH 5.5 |
