8HTY
Candida boidinii Formate Dehydrogenase Crystal Structure at 1.4 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-31 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.132, 68.959, 109.792 |
| Unit cell angles | 78.14, 89.48, 81.11 |
Refinement procedure
| Resolution | 21.830 - 1.400 |
| R-factor | 0.1675 |
| Rwork | 0.167 |
| R-free | 0.19040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dna |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.422 |
| Data reduction software | CrysalisPro (1.171.42.35a) |
| Data scaling software | CrysalisPro (1.171.42.35a) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.750 | 1.510 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Number of reflections | 301717 | 18691 |
| <I/σ(I)> | 11.74 | |
| Completeness [%] | 98.8 | 86 |
| Redundancy | 151.46 | |
| CC(1/2) | 0.999 | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% (w/v) PEG 5000 MME, 100 mM MES/Sodium hydroxide pH 6.5, 200 mM Ammonium sulfate |
