8HT3
Crystal structure of immunoglobulin new antigen receptor variable domain from Okamejei kenojei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-14 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.901, 72.213, 70.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.050 - 2.500 |
| R-factor | 0.2005 |
| Rwork | 0.195 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.514 |
| Data reduction software | CrysalisPro |
| Data scaling software | XDS |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.050 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.028 | |
| Rmeas | 0.031 | |
| Rpim | 0.013 | |
| Number of reflections | 4028 | 1866 |
| <I/σ(I)> | 61.28 | |
| Completeness [%] | 98.9 | 97.92 |
| Redundancy | 5.2 | |
| CC(1/2) | 1.000 | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M Lithium sulfate monohydrate/0.1 M Tris pH 8.0/26% w/v PEG4000 |
