8HST
The structure of rat beta-arrestin1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.285, 72.484, 116.388 |
| Unit cell angles | 90.00, 98.78, 90.00 |
Refinement procedure
| Resolution | 38.620 - 2.660 |
| R-factor | 0.1868 |
| Rwork | 0.184 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (v1.19.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.710 |
| High resolution limit [Å] | 2.660 | 7.210 | 2.660 |
| Rmerge | 0.984 | 0.058 | 0.368 |
| Total number of observations | 201036 | ||
| Number of reflections | 57442 | 2867 | 2741 |
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 99.6 | 99.3 | 95.4 |
| Redundancy | 3.5 | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 5% (w/v) PEG 8000, 100mM Imidazole/ Hydrochloric acid pH 8.0, 100mM Calcium acetate |
