8HRO
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-10-31 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.730, 117.348, 75.815 |
Unit cell angles | 90.00, 116.88, 90.00 |
Refinement procedure
Resolution | 44.810 - 2.590 |
R-factor | 0.19025 |
Rwork | 0.187 |
R-free | 0.27154 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dbv |
RMSD bond length | 0.006 |
RMSD bond angle | 1.408 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.590 | 7.050 | 2.590 |
Rmerge | 0.111 | 0.069 | 0.274 |
Number of reflections | 20397 | 2137 | 1768 |
<I/σ(I)> | 14.4 | ||
Completeness [%] | 92.8 | 99.3 | 84.5 |
Redundancy | 2.6 | 3.6 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 8K, HEPES |