8HRO

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-31
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	90.730, 117.348, 75.815
Unit cell angles	90.00, 116.88, 90.00

Refinement procedure

Resolution	44.810 - 2.590
R-factor	0.19025
Rwork	0.187
R-free	0.27154
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dbv
RMSD bond length	0.006
RMSD bond angle	1.408
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.590	7.050	2.590
Rmerge	0.111	0.069	0.274
Number of reflections	20397	2137	1768
<I/σ(I)>	14.4
Completeness [%]	92.8	99.3	84.5
Redundancy	2.6	3.6	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 8K, HEPES