8HR2
Ternary Crystal Complex Structure of RBD with NB1B5 and NB1C6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 89.522, 89.522, 125.821 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.850 - 1.940 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ch5 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.067 |
Refinement software | PHENIX (v1.20.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.000 | 2.040 |
High resolution limit [Å] | 1.940 | 1.940 |
Number of reflections | 39139 | 3773 |
<I/σ(I)> | 24.7 | |
Completeness [%] | 100.0 | |
Redundancy | 13.5 | |
CC(1/2) | 1.000 | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293.15 | 0.2 mM HEPES pH7.0;12% PEG3350 |