8HQX
The complex structure of COPI cargo sorting module with TAT-WDM peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.863, 50.831, 72.643 |
| Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
| Resolution | 36.495 - 1.538 |
| R-factor | 0.2248 |
| Rwork | 0.224 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.739 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16.3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.538 | 1.550 |
| Rmerge | 0.086 | 0.414 |
| Number of reflections | 28943 | 240 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 281.15 | 0.1 M MES, pH 6.5 and 20% PEG3350 |
