8HQV
The complex structure of COPI cargo sorting module with HCoV-OC43 Spike KTSHxx sorting motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.930, 51.273, 85.256 |
| Unit cell angles | 90.00, 101.61, 90.00 |
Refinement procedure
| Resolution | 42.033 - 1.398 |
| R-factor | 0.1876 |
| Rwork | 0.187 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-3000 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16.3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.398 | 1.400 |
| Rmerge | 0.089 | |
| Number of reflections | 56731 | 4767 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 80.2 | 80.2 |
| Redundancy | 3.7 | 3.2 |
| CC(1/2) | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 281.15 | 0.2 M MgSO4 and 20% PEG3350 |
