8HQT
The complex structure of COPI cargo sorting module with SARS-CoV-2 Spike KxHxx sorting motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.892, 51.051, 85.101 |
| Unit cell angles | 90.00, 101.62, 90.00 |
Refinement procedure
| Resolution | 41.903 - 1.800 |
| R-factor | 0.2098 |
| Rwork | 0.209 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.804 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.106 | 0.645 |
| Rmeas | 0.441 | |
| Rpim | 0.332 | |
| Number of reflections | 28821 | 1411 |
| <I/σ(I)> | 24.2 | 1.1 |
| Completeness [%] | 98.3 | 96.5 |
| Redundancy | 3.8 | 3.4 |
| CC(1/2) | 0.960 | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289.15 | 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM DTT. |
