8HQP

Crystal structure of AbHheG mutant from Acidimicrobiia bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	81.247, 87.210, 121.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.820 - 1.620
R-factor	0.2084
Rwork	0.207
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wkq
RMSD bond length	0.009
RMSD bond angle	1.405
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.620	1.620
Number of reflections	110244	8063
<I/σ(I)>	22.97	2.85
Completeness [%]	100.0	100
Redundancy	14.22	14.68
CC(1/2)	0.990	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20% PEG 3350, 0.1 M Bis-Tris pH 6.5, 2% v/v Tacsimate pH6.0