8HQP
Crystal structure of AbHheG mutant from Acidimicrobiia bacterium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.247, 87.210, 121.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.820 - 1.620 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wkq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.405 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Number of reflections | 110244 | 8063 |
| <I/σ(I)> | 22.97 | 2.85 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.22 | 14.68 |
| CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20% PEG 3350, 0.1 M Bis-Tris pH 6.5, 2% v/v Tacsimate pH6.0 |
