8HQL

Crystal structure of mouse SNX25 PX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 61 2 2
Unit cell lengths	92.680, 92.680, 449.521
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.000 - 2.400
R-factor	0.20074
Rwork	0.199
R-free	0.24186
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.877
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.970	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.078	0.780
Rmeas	0.084	0.832
Rpim	0.029	0.285
Total number of observations	360104	35765
Number of reflections	46187	4398
<I/σ(I)>	16.6	2.8
Completeness [%]	99.9
Redundancy	7.8	8.1
CC(1/2)	0.995	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.8 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.8