8HQ8
Bry-LHCII homotrimer of Bryopsis corticulans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.720, 91.610, 169.220 |
| Unit cell angles | 90.00, 103.52, 90.00 |
Refinement procedure
| Resolution | 39.900 - 2.600 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18_3861: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.062 | 0.062 |
| Rpim | 0.026 | |
| Number of reflections | 68491 | 1284 |
| <I/σ(I)> | 13.84 | |
| Completeness [%] | 94.3 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES, PEG 1000, magnesium chloride,betaine, Cymal3 |
