8HQ4
B27 in complex with CRM1-Ran-RanBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-10-24 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.080, 106.080, 304.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.060 - 2.120 |
R-factor | 0.1879 |
Rwork | 0.187 |
R-free | 0.21020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.432 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.060 | 32.060 | 2.180 |
High resolution limit [Å] | 2.120 | 9.480 | 2.120 |
Rmerge | 0.133 | 0.079 | 2.140 |
Rmeas | 0.136 | 0.081 | 2.180 |
Rpim | 0.027 | 0.017 | 0.412 |
Total number of observations | 2605400 | 26161 | 198874 |
Number of reflections | 99275 | 1272 | 7240 |
<I/σ(I)> | 16.5 | 37.5 | 2.1 |
Completeness [%] | 99.9 | 97.5 | 100 |
Redundancy | 26.2 | 20.6 | 27.5 |
CC(1/2) | 0.999 | 0.997 | 0.677 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.12 M Monosaccharides (20 mM D-Glucose; 20 mM D-Mannose; 20 mM D-Galactose; 20 mM L-Fucose; 20 mM D-Xylose; 20 mM N-Acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (sodium HEPES and MOPS), and 50 % Precipitant Mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000) |