8HPI

Crystal structure of Tyrosinase from Priestia megaterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-03
Detector	Bruker PHOTON II
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	104.950, 104.950, 137.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.980 - 2.540
R-factor	0.20238
Rwork	0.200
R-free	0.24909
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.392
Data scaling software	Aimless

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	2.650
High resolution limit [Å]	2.540	2.540
Number of reflections	39392	3
<I/σ(I)>	4.08
Completeness [%]	99.4
Redundancy	4.5
CC(1/2)	0.993	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	PEG 4000, Zinc acetate dehydrate, Sodium acetate trihydrate