8HPE

Crystal structure of Leucine dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-14
Detector	Bruker PHOTON II
Wavelength(s)	1.54184
Spacegroup name	P 4 21 2
Unit cell lengths	120.949, 120.949, 177.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.240 - 3.220
R-factor	0.23571
Rwork	0.233
R-free	0.29529
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.527
Data scaling software	Aimless
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	3.480
High resolution limit [Å]	3.220	3.220
Rmerge	0.302	1.200
Number of reflections	22022	4448
<I/σ(I)>	8.7
Completeness [%]	99.9
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% glycerol, 1.5M ammonium sulfate