8HO6

ScRIPK WT

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	69.170, 72.350, 79.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.738 - 2.100
R-factor	0.2059
Rwork	0.203
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tos
RMSD bond length	0.007
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.060	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.138
Number of reflections	23842	23842
<I/σ(I)>	13	1.7
Completeness [%]	99.7
Redundancy	12.79
CC(1/2)	0.998	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400, 0.19 mM Cymal-7, 10% v/v Polyethylene glycol 3350, 0.18 mM GDN