8HNO

Archaeal transcription factor Wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	102.686, 102.686, 90.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.920 - 2.840
R-factor	0.2228
Rwork	0.222
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.097
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.890
High resolution limit [Å]	2.840	2.840
Rmerge	0.076	0.414
Number of reflections	11845	545
<I/σ(I)>	15.571
Completeness [%]	98.9	97
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	30% polyethylene glycol (PEG) 400, 100 mM HEPES pH 7.5, and 200 mM sodium chloride