8HNE

Crystal structure of the ancestral GH19 chitinase Anc4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	32.140, 76.240, 78.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.470 - 1.130
R-factor	0.241
Rwork	0.241
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.470	1.170
High resolution limit [Å]	1.130	1.130
Rmerge	0.172	0.230
Rmeas	0.243
Rpim	0.172
Number of reflections	73261	6976
<I/σ(I)>	4.14
Completeness [%]	99.6
Redundancy	2
CC(1/2)	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		316	15% (w/v) polyethylene glycol monomethyl ether 2000, 0.1M MES monohydrate pH 6.0