8HN6

Crystal structure of monoclonal antibody complexed with SARS-CoV-2 RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	98.680, 105.730, 209.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.430 - 2.070
R-factor	0.193
Rwork	0.192
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.097
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.650	2.120
High resolution limit [Å]	2.070	2.070
Number of reflections	66979	4877
<I/σ(I)>	18.7
Completeness [%]	99.9
Redundancy	12.3
CC(1/2)	0.999	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	PEG 3350,0.1 M HEPES,0.2 M LProline