8HN3
Soluble domain of cytochrome c-556 from Chlorobaculum tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 85.024, 85.024, 123.932 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.820 - 1.650 |
| R-factor | 0.15427 |
| Rwork | 0.153 |
| R-free | 0.17357 |
| Structure solution method | SAD |
| RMSD bond length | 0.033 |
| RMSD bond angle | 3.311 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.056 | 0.240 |
| Number of reflections | 32195 | 3113 |
| <I/σ(I)> | 95.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 20.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 0.1 M sodium acetate trihydrate pH 4.4, 1.8 M ammonium sulfate |
