8HN0

Crystal structure of N-terminal fragment (20-132aa) of human SCARF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.014, 50.702, 110.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.710 - 2.200
R-factor	0.2226
Rwork	0.219
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.068
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2-3874-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.072	0.602
Number of reflections	16105	1567
<I/σ(I)>	33
Completeness [%]	99.8
Redundancy	9.9
CC(1/2)	0.994	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	20% (w/v) polyethylene glycol 3350, 0.2 M ammonium sulfate, 0.1 M Bis-Tris (pH 5.5)