8HMX
WDR5 in complex with histone H3Q5his peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-10-14 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.187, 46.857, 65.297 |
| Unit cell angles | 90.00, 112.58, 90.00 |
Refinement procedure
| Resolution | 31.960 - 1.700 |
| R-factor | 0.1698 |
| Rwork | 0.168 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.871 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.970 | 0.091 | 0.482 |
| Total number of observations | 122911 | ||
| Number of reflections | 34866 | 3388 | 3268 |
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 96.3 | 98.4 | 96.1 |
| Redundancy | 1.9 | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium citrate tribasic dihydrate (pH 5.5), 22% polyethylene glycol (PEG) 3350, and 0.1% n-Octyl-beta-D-glucoside |
