8HMN
Crystal structure of GAPDH complexed with arsenate from Lactiplantibacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-31 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.850, 123.964, 132.859 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.590 - 2.160 |
| R-factor | 0.21835 |
| Rwork | 0.216 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.496 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.490 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.088 | 0.908 |
| Rmeas | 0.104 | |
| Rpim | 0.056 | 0.574 |
| Number of reflections | 69658 | 5267 |
| <I/σ(I)> | 13.1 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.5 | 6.5 |
| CC(1/2) | 0.998 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25%(w/v) PEG 1500, 0.1 M MIB buffer |
