8HML
Co-crystal structure of the C terminal DNA binding domain of Saccharopolyspora erythraea GlnR in complex with its conserved promoter DNA in 2.95 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.279, 46.279, 366.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.842 - 2.950 |
| R-factor | 0.2617 |
| Rwork | 0.256 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 10.000 | 2.380 |
| High resolution limit [Å] | 2.340 | 6.350 | 2.340 |
| Rmerge | 0.081 | 1.720 | |
| Number of reflections | 9134 | 1238 | 794 |
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 90.7 | 98.3 | 78.3 |
| Redundancy | 5.1 | 7.2 | 3.6 |
| CC(1/2) | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M Bis-tris pH6.0, 20% PEG3350 |
