8HMH

The closed state of RGLG2-VWA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979183
Spacegroup name	P 41
Unit cell lengths	62.960, 62.960, 155.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.873 - 2.560
R-factor	0.1896
Rwork	0.187
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.027
RMSD bond angle	1.462
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.873	2.630
High resolution limit [Å]	2.560	2.560
Number of reflections	19226	962
<I/σ(I)>	11.3	2.1
Completeness [%]	99.0
Redundancy	13.9
CC(1/2)	0.994	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	200 mM magnesium chloride, 30% PEG 4000, 100 mM Tris-HCl, PH8.5