8HM5
Epoxide hydrolase from Caballeronia sordidicola PAMC 26510
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2020-08-05 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.470, 81.210, 79.830 |
Unit cell angles | 90.00, 93.11, 90.00 |
Refinement procedure
Resolution | 43.410 - 2.430 |
R-factor | 0.1728 |
Rwork | 0.171 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.224 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.210 | 2.560 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.070 | 0.177 |
Number of reflections | 20365 | 2022 |
<I/σ(I)> | 10.5 | 6.1 |
Completeness [%] | 97.7 | |
Redundancy | 4.5 | |
CC(1/2) | 0.993 | 0.968 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.2 M magnesium formate and 20% (w/v) PEG 3350 |