8HM2

Crystal structure of human ubiquitin-like protein from bacteroides fragilis c terminal cysteine mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	38.537, 38.537, 177.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.070 - 1.340
R-factor	0.2562
Rwork	0.256
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.007
RMSD bond angle	1.067
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.070	1.388
High resolution limit [Å]	1.340	1.340
Number of reflections	30343	2397
<I/σ(I)>	13.5
Completeness [%]	96.8
Redundancy	20
CC(1/2)	0.994	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	2.8	291	0.1M citric acid pH 2.8, 1.6 M ammonium sulfate