8HM1

crystal structure of human ubiquitin-like protein from Bacteroides fragilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	38.380, 38.380, 190.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.860 - 1.290
R-factor	0.25
Rwork	0.249
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.018
RMSD bond angle	1.936
Data reduction software	XDS (1.19.2_4158)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.860	1.336
High resolution limit [Å]	1.290	1.290
Number of reflections	36628	3124
<I/σ(I)>	14.1	1.7
Completeness [%]	98.1
Redundancy	20
CC(1/2)	0.998	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M sodium citrate pH 3.0, 3.4 M cesium chloride