8HLS
Crystal structure of the sialidase Am1547 from A.muciniphila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 83 |
| Detector technology | PIXEL |
| Collection date | 2020-03-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.160, 56.771, 145.932 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 21.640 - 2.040 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.22260 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.938 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.640 | 2.115 |
| High resolution limit [Å] | 2.040 | 2.042 |
| Rmeas | 0.079 | |
| Rpim | 0.056 | |
| Number of reflections | 140126 | 6090 |
| <I/σ(I)> | 12.75 | |
| Completeness [%] | 97.0 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Magnesium chloride,Bis-Tris , PEG 3350 |
