8HLO
Crystal structure of ASAP1-SH3 and MICAL1-PRM complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 62 |
Unit cell lengths | 45.181, 45.181, 59.391 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.128 - 1.168 |
R-factor | 0.1143 |
Rwork | 0.111 |
R-free | 0.14240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.317 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASES |
Refinement software | PHENIX (v1.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.128 | 1.210 |
High resolution limit [Å] | 1.168 | 1.170 |
Rmerge | 0.041 | |
Number of reflections | 21440 | 1130 |
<I/σ(I)> | 11.32 | |
Completeness [%] | 91.7 | |
Redundancy | 4.9 | |
CC(1/2) | 0.990 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 289.15 | 0.1M HEPES, pH 7.5, 1.4M Sodium citrate tribasic dihydrate |