8HLM
Crystal structure of p53/BCL2 fusion complex (complex 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-19 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.610, 70.336, 127.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.205 - 2.522 |
R-factor | 0.2394 |
Rwork | 0.234 |
R-free | 0.29010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1TUP/6QGG |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.15.2_3472) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.580 | 2.612 |
High resolution limit [Å] | 2.520 | 2.522 |
Rmerge | 0.088 | 0.911 |
Rpim | 0.308 | |
Number of reflections | 13872 | 1383 |
<I/σ(I)> | 0.0935 | |
Completeness [%] | 96.2 | |
Redundancy | 9.1 | |
CC(1/2) | 0.999 | 0.924 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 0.1 M Imidazole, PEG8K 15-21% |