8HL9
Crystal structure of Galectin-8 C-CRD with part of linker
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 52.900, 52.900, 126.749 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.620 - 1.600 |
R-factor | 0.2166 |
Rwork | 0.213 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.910 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX (v1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.620 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.058 | 0.520 |
Number of reflections | 24410 | 1173 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 99.3 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Hepes pH 7.0, 0.2M MgCl2, 30% (W/V) PEG 3350 |