8HKW
Crystal structure of importin-alpha3 bound to the 53BP1 nuclear localization signal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-03-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 95.703, 79.600, 117.440 |
| Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
| Resolution | 27.338 - 1.900 |
| R-factor | 0.2017 |
| Rwork | 0.201 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.535 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (v1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.340 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 68772 | 4433 |
| <I/σ(I)> | 4.9 | 0.7 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.993 | 0.338 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Mother liquor contained PEG3350 and lithium nitrate. Crystal was grown in the presence of a synthetic peptide (amino acid sequence: GTSFSGRKIKTAVRRRK) that corresponds to human Nup153 residues 1459-1475). |
