8HKH
Crystal structure of the LC/A1-DARPin18 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.560, 59.630, 102.180 |
| Unit cell angles | 74.98, 89.90, 73.15 |
Refinement procedure
| Resolution | 49.188 - 2.700 |
| R-factor | 0.2639 |
| Rwork | 0.262 |
| R-free | 0.30610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 39460 | 2962 |
| <I/σ(I)> | 4.49 | 1.17 |
| Completeness [%] | 93.4 | 94.2 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.976 | 0.452 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5 28 % w/v Jeffamine ED-2003 |
