8HKH

Crystal structure of the LC/A1-DARPin18 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	59.560, 59.630, 102.180
Unit cell angles	74.98, 89.90, 73.15

Refinement procedure

Resolution	49.188 - 2.700
R-factor	0.2639
Rwork	0.262
R-free	0.30610
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.190	2.620
High resolution limit [Å]	2.550	2.550
Number of reflections	39460	2962
<I/σ(I)>	4.49	1.17
Completeness [%]	93.4	94.2
Redundancy	3.7	3.8
CC(1/2)	0.976	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7.5 28 % w/v Jeffamine ED-2003