8HKG
Crystal structure of glycosidic hydrolase family 10 (GH10) xylanase XynA contains an additional proline-rich sequence in the C-terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.922, 99.461, 87.833 |
Unit cell angles | 90.00, 100.91, 90.00 |
Refinement procedure
Resolution | 24.240 - 2.560 |
R-factor | 0.2192 |
Rwork | 0.215 |
R-free | 0.30830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.111 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.240 | 2.655 |
High resolution limit [Å] | 2.563 | 2.563 |
Rmerge | 0.088 | |
Number of reflections | 18264 | |
<I/σ(I)> | 6.57 | |
Completeness [%] | 98.9 | |
Redundancy | 1.9 | |
CC(1/2) | 0.969 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 30% (w/v) PEG 4000, 0.2M ammonium acetate, 0.1M sodium acetate trihydrate |