8HK0

Crystal structure of Fic32-33 complex from Streptomyces ficellus NRRL 8067

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 4 21 2
Unit cell lengths	137.080, 137.080, 148.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.440 - 2.290
R-factor	0.186
Rwork	0.183
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.139
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.600	2.350
High resolution limit [Å]	2.290	2.290
Number of reflections	63948	4640
<I/σ(I)>	16.5
Completeness [%]	100.0
Redundancy	13.7
CC(1/2)	0.999	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium cacodylate and 36% w/v PEG2000 MME (pH 6.5)