8HJQ

Crystal structure of glycosyltransferase SgUGT94-289-3 in apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	63.901, 81.433, 90.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.410 - 3.050
R-factor	0.2237
Rwork	0.222
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jtd
RMSD bond length	0.011
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.410	3.159
High resolution limit [Å]	3.050	3.050
Rmerge	0.110
Number of reflections	9376	900
<I/σ(I)>	13.23
Completeness [%]	99.6
Redundancy	6.1
CC(1/2)	0.997	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	Tris-HCl, polyethylene glycol 3350, NaCl, Polypropylene glycol P400.