8HJJ

Anti-CRISPR protein AcrIC9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 31 1 2
Unit cell lengths	69.410, 69.410, 89.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.480 - 2.400
R-factor	0.2171
Rwork	0.214
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.480	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.289	2.096
Number of reflections	9785	968
<I/σ(I)>	15.03
Completeness [%]	99.7
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2 M Ammonium sulfate, 0.1 M cacodylate pH 5.6, 0.2 M NaCl