8HJF
Crystal structure of glycosyltransferase SgUGT94-289-3 in complex with M5, state 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.955, 76.814, 81.459 |
| Unit cell angles | 90.00, 93.25, 90.00 |
Refinement procedure
| Resolution | 19.660 - 2.000 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jtd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.972 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.660 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 64729 | 6412 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.987 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | Tris-HCl, polyethylene glycol 3350, NaCl, Polypropylene glycol P400. |
