8HJA

The crystal structure of syn_CdgR-(c-di-GMP) from Synechocystis sp. PCC 6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2019-12-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	96.327, 55.978, 91.692
Unit cell angles	90.00, 111.97, 90.00

Refinement procedure

Resolution	47.480 - 2.380
R-factor	0.23076
Rwork	0.224
R-free	0.28568
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.707
Data scaling software	HKL-3000
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.350	2.400
Number of reflections	18257	894
<I/σ(I)>	27.43
Completeness [%]	99.4	95.9
Redundancy	6.2	5.5
CC(1/2)	0.978	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	21% PEG 3350, 0.2 M sodium chloride, 0.1 M Bis-Tris pH 6.8