8HJA
The crystal structure of syn_CdgR-(c-di-GMP) from Synechocystis sp. PCC 6803
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.327, 55.978, 91.692 |
Unit cell angles | 90.00, 111.97, 90.00 |
Refinement procedure
Resolution | 47.480 - 2.380 |
R-factor | 0.23076 |
Rwork | 0.224 |
R-free | 0.28568 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.707 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.20.1-4487) |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.350 | 2.400 |
Number of reflections | 18257 | 894 |
<I/σ(I)> | 27.43 | |
Completeness [%] | 99.4 | 95.9 |
Redundancy | 6.2 | 5.5 |
CC(1/2) | 0.978 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 21% PEG 3350, 0.2 M sodium chloride, 0.1 M Bis-Tris pH 6.8 |