8HJ9
cryoEM structure of glutamate dehydrogenase from Thermococcus profundus in complex with NADP
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | JEOL CRYO ARM 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.12 |
Number of Particle | 3037558 |
Software | RELION,Warp,Coot,PHENIX |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |