8HIV
The structure of apo-SoBcmB with Fe(II) and AKG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 101.677, 101.677, 130.922 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.783 - 2.200 |
| R-factor | 0.188791274986 |
| Rwork | 0.187 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.937 |
| Data reduction software | xia2 |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.030 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.111 | 0.942 |
| Rmeas | 0.115 | 0.979 |
| Rpim | 0.032 | 0.264 |
| Number of reflections | 20954 | 1769 |
| <I/σ(I)> | 17.2 | 5.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 13.8 |
| CC(1/2) | 0.998 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1M Potassium chloride, 0.1M HEPES pH 7, 1M Ammonium sulfate |
