8HIG
Co-crystal structure of C-terminal DNA binding domain of Saccharopolyspora erythraea GlnR in complex with its cognate promoter DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 46.279, 46.279, 366.859 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.842 - 2.329 |
R-factor | 0.2654 |
Rwork | 0.259 |
R-free | 0.32470 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.329 | 2.340 |
Rmerge | 1.720 | |
Number of reflections | 19059 | 569 |
<I/σ(I)> | 11.146 | 2.034 |
Completeness [%] | 90.7 | 78.3 |
Redundancy | 5.1 | |
CC(1/2) | 0.900 | 0.107 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1M Bis-Tris pH5.7, 25% PEG3350 |