8HIB
The crystal structure of Pygo2-LDB1-SSBP2 triple complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 104.499, 104.499, 252.274 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.920 - 2.450 |
R-factor | 0.2146 |
Rwork | 0.213 |
R-free | 0.24620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6tyd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.19.2-4158) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.520 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 30828 | 2491 |
<I/σ(I)> | 44.86 | |
Completeness [%] | 100.0 | |
Redundancy | 12.7 | |
CC(1/2) | 0.998 | 0.614 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM Li2SO4, 100 mM sodium citrate tribasic dihydrate pH 5.6, 1% v/v PEG400, and 10 mM DTT |