8HHJ
Crystal structure of the MafB2-CTMGI-2B16B6/MafI2MGI-2B16B6 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-30 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9734 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.667, 39.792, 65.547 |
Unit cell angles | 90.00, 109.16, 90.00 |
Refinement procedure
Resolution | 28.903 - 1.500 |
R-factor | 0.1822 |
Rwork | 0.180 |
R-free | 0.20820 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.808 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AutoSol |
Refinement software | PHENIX (v1.11) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 30286 | 1523 |
<I/σ(I)> | 48.3 | |
Completeness [%] | 97.9 | |
Redundancy | 7.3 | |
CC(1/2) | 0.999 | 0.970 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.25 - 0.3 M magnesium formate, 25 - 30% polyethylene glycol (PEG) 3350 |