8HHH
The bacterial divisome protein complex FtsB-FtsL-FtsQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-12-22 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 100.290, 54.320, 179.261 |
| Unit cell angles | 90.00, 94.51, 90.00 |
Refinement procedure
| Resolution | 34.690 - 3.300 |
| R-factor | 0.277 |
| Rwork | 0.276 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8hhg |
| Data reduction software | iMOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.330 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.074 | 0.616 |
| Number of reflections | 14819 | 1476 |
| <I/σ(I)> | 9.25 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris pH 8.0, 20-35% PEG400 |
